A number of conditions that may cause vitamin B deficiency have been reported previously. Hyperhomocysteinemia is also associated with increased mortality in the elderly. A number of studies have suggested that endothelial dysfunction and oxidation of lowdensity lipoprotein is linked to hyperhomocysteinemia. Vitamin B deficient patients are usually treated with high oral or parenteral doses of cobalamin for months. To the best of our knowledge, data on acute responses to aminothiols and vitamin B, and mean cell volume to vitamin B supplementation have not been reported earlier.In the present study, we have investigated the acute changes in aminothiols, folate and sMMA concentrations and changes in hematological parameters during weeks of intramuscular cyanocobalamin administration in vitamin B deficient patients.The inclusion criteria for the patients to participate in this study were: low concentration of serum vitamin B. Patients were <a href="http://www.targetmol.com/compound/Prilocaine">Targetmol's
Prilocaine</a> contacted and asked if they were willing to participate in the study.Among the patients, patient was finally diagnosed with cancer, with anemia, with hypothyroidism and had cardiovascular disease.The remaining patients were diagnosed with vitamin B deficiency.The blood samples were collected at general practitioner offices or in the laboratory of medical biochemistry.Since the main focus of this study was to explore acute intraindividual responses of biochemical and hematological parameters to vitamin B administration, information on nutrition status and data on body mass index were not collected.Blood samples were collected after an overnight fast from all the participants before and, and days after cobalamin administration.Plasma and serum samples were stored at C until blood sample analysis.Written informed consent was obtained from all the participants, and ethical committee III approved the study.The monobromobimaneaminothiol conjugates were eluted and separated by ion pair HPLC and these were detected with a fluorescence detector.The concentration of serum folate, erythrocyte folate and serum vitamin B were measured according to the methods described previously. Similarly, the concentration of serum cobalamin was not significantly higher in the female patients than in the male patients. Therefore, the data from female and male patients were combined.The sfolate concentration decreased, but the difference was not significant. Table shows the blood parameter concentrations in vitamin B deficient patients before and after cyanocobalamin administration.Serum vitamin B concentration was significantly increased after the day vitamin therapy. At the same time, the concentration of sfolate was significantly decreased after the week vitamin therapy. Table also demonstrates that a significant decrease in the sfolate concentration could already be detected day after the first injection of cobalamin folate was decreased after day of therapy, but thereafter values were increased compared to the baseline concentration up to the last sampling at weeks, but no significant difference was found. The concentration of sMMA decreased progressively and consecutively from days to of the vitamin therapy. MCV was significantly decreased after weeks of vitamin therapy.We here report that vitamin B deficient patients showed significant changes in biochemical parameters after only day of intramuscular cobalamin administration whereas changes in hematological parameters were first observed after days.A number of studies have demonstrated that oral doses of vitamin B given to vitamindeficient patients may be as effective as vitamin B administered intramuscularly to these patients.