Resultant peaks were then analysed for their mean peak area ratios and concentrations.Mean and standard deviation values were calculated and then <a href="http://www.targetmol.com/compound/Cholic-Acid">sell
Cholic Acid</a> deduced LOQ and LOD values for cyanocobalamin using specific equations.The relative standard deviation as a fractional error expressed as a percentage was deduced for the analysis.RSD value of less than is achievable for HPLC method for analysis.Their colour, acidity and volume of the contents of each is tabulated below.The quality assurances of these products are indispensable in maintaining a drugs intergrity and quality over its shelflife.The three main samples employed for the project were cyanocobalamin and ascorbic acid pure powders and cyanocobalamin injections.It was necessary to check the quality of both cyanocobalamin and ascorbic acid pure powders using the pharmacopoeia prescribed methods.This involves checking the quality by both the identification and quantitative assay for percentage content.The pH range employed revealed that at pH there was substantial quantity of cyanocobalamin in solution as indicated in the absorbance versus pH graph. This is due to the fact that at pH cyanocobalamim is fully unionized and undegradable into other B forms. that at pH cyanocobalamin has the highest concentration in solution.The effect of sunlight on the stability of aqueous solutions of cyanocobalamin was also monitored over a six hour period.Commercial samples were therefore stored in a way that protected them from light to avoid degradation.Thermal stability of cyanocobalamin was also studied using the commercial samples over a temperature ranging from to oC.It was observed that the injections were quite stable to heat over the entire temperature range employed. The maximum tropical temperature is between to C which therefore makes the product stable under normal tropical temperature conditions.Mostly, the end products of thermal degradation of cyanocobalamin are toxic gases such as carbon monoxides, nitrogen dioxide, oxides of cobalt and phosphorus; however, these products are likely to be released at high temperatures.The melting point of pure cyanocobalamin is greater than oC and hence these products are not likely to be released under normal tropical temperatures.No substantial decrease in absorbance was therefore obtained during the studies.This could be due to the fact that the injections were kept in heat proof ampoules coupled with the fact that the temperature range employed could not have a substantial conductive effect on the ampoules.A reverse phase hplc coloumn was chosen because cyanocobalamin has a lot of polar groups on its structure and therefore does not retain much longer on the column.UV detection was chosen for the method because of the presence of extensive chromophoric system in cyanacobalamin.The choice of mobile phase was important for successful liquid chromatography.In this work, the organic phase was initially changed while the aqueous medium was fixed.Various mobile phase compositions comprising of watermethanol at vv employed.In each composition, a quantity of the cyanocobalamin and a constant amount of internal standard were added.The composition was injected whilst varying the conditions. Mobile phase composition of vv was found most suitable because it gave the best resolution; however, the separation of the drug and the internal standard was very poor.